Purification

Target

IDP90567  

Purification ID

A4758  

Expression

M9 minimal + semet (Nov 10, 2009)  

Experiment Date Start

Nov 13, 2009  

Experiment Date End

Nov 15, 2009  

Person

Natalia Maltseva  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, SEC::TEV cleavage::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

cleavage unsuccessful  

SeMet Status

semet  

Covalent Modification

 

Final Buffer

20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  

Final Concentration (mg/mL)

73  

Final Volume (μL)

1500  

Shipped To Lab Id

ANL  

Shipped Date

Nov 15, 2009  

Inserted

Jul 29, 2011