Purification

Target

IDP02693  

Purification ID

A5335  

Expression

M9 minimal + semet (Mar 07, 2009)  

Experiment Date Start

Oct 08, 2009  

Experiment Date End

Oct 10, 2009  

Person

Rory Mulligan  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

cleaved  

SeMet Status

semet  

Covalent Modification

 

Final Buffer

50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  

Final Concentration (mg/mL)

79  

Final Volume (μL)

1200  

Shipped To Lab Id

ANL  

Shipped Date

Oct 10, 2009  

Inserted

Jul 29, 2011