Purification

Target

IDP02448  

Purification ID

A5143  

Expression

M9 minimal + semet (Jun 15, 2009)  

Experiment Date Start

Jul 08, 2009  

Experiment Date End

Jul 10, 2009  

Person

Min Zhou  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, SEC, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

cleaved  

SeMet Status

semet  

Covalent Modification

 

Final Buffer

50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  

Final Concentration (mg/mL)

84  

Final Volume (μL)

800  

Shipped To Lab Id

ANL  

Shipped Date

Jul 10, 2009  

Inserted

Jul 29, 2011