Purification

Target

IDP00573  

Purification ID

A4597  

Expression

M9 minimal + semet (Feb 18, 2009)  

Experiment Date Start

Feb 23, 2009  

Experiment Date End

Feb 25, 2009  

Person

Natalia Maltseva  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

cleaved  

SeMet Status

semet  

Covalent Modification

 

Final Buffer

20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  

Final Concentration (mg/mL)

90  

Final Volume (μL)

1000  

Shipped To Lab Id

ANL  

Shipped Date

Feb 25, 2009  

Inserted

Jul 29, 2011