Purification

Target
IDP01484  
Purification ID
A3980  
Expression
M9 minimal + semet (Nov 04, 2008)  
Experiment Date Start
Nov 10, 2008  
Experiment Date End
Nov 12, 2008  
Person
Minyi Gu  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, SEC, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  
Affinity Tag Status
cleaved  
SeMet Status
semet  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
92  
Final Volume (μL)
800  
Shipped To Lab Id
ANL  
Shipped Date
Nov 12, 2008  
Inserted
Jul 29, 2011  

Related Crystallization Drops

Experiment
Date		 Person Lab Protocol Status Method Drop
Precipitant
Contents		 Screen Screen
Component		 pH Plate
Type