Purification

Target

IDP00538  

Purification ID

A4133  

Expression

LB (Jul 16, 2008)  

Experiment Date Start

Jul 21, 2008  

Experiment Date End

Jul 23, 2008  

Person

Min Zhou  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, SEC, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

cleaved  

SeMet Status

native  

Covalent Modification

 

Final Buffer

50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  

Final Concentration (mg/mL)

99  

Final Volume (μL)

800  

Shipped To Lab Id

ANL  

Shipped Date

Jul 23, 2008  

Inserted

Jul 29, 2011