Purification

Target

IDP90540  

Purification ID

A3757  

Expression

M9 minimal (Nov 11, 2009)  

Experiment Date Start

Dec 02, 2009  

Experiment Date End

Dec 04, 2009  

Person

Minyi Gu  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

cleaved  

SeMet Status

native  

Covalent Modification

 

Final Buffer

20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  

Final Concentration (mg/mL)

118  

Final Volume (μL)

500  

Shipped To Lab Id

ANL  

Shipped Date

Dec 04, 2009  

Inserted

Jul 29, 2011