Purification

Target

IDP90506  

Purification ID

A4068  

Expression

2xYT (Oct 18, 2010)  

Experiment Date Start

Oct 27, 2010  

Experiment Date End

Oct 29, 2010  

Person

Minyi Gu  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, SEC, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

cleaved  

SeMet Status

native  

Covalent Modification

 

Final Buffer

20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  

Final Concentration (mg/mL)

121  

Final Volume (μL)

400  

Shipped To Lab Id

ANL  

Shipped Date

Oct 29, 2010  

Inserted

Jul 29, 2011