Purification

Target

IDP01038  

Purification ID

A3644  

Expression

M9 minimal (Nov 04, 2008)  

Experiment Date Start

Nov 10, 2008  

Experiment Date End

Nov 12, 2008  

Person

Minyi Gu  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, SEC::TEV cleavage::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

cleavage unsuccessful  

SeMet Status

native  

Covalent Modification

 

Final Buffer

20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  

Final Concentration (mg/mL)

130  

Final Volume (μL)

980  

Shipped To Lab Id

ANL  

Shipped Date

Nov 12, 2008  

Inserted

Jul 29, 2011