Center for Structural Genomics of Infectious Diseases

Purification

Target: IDP01334
Purification ID: A4441
Expression: M9 minimal (Jul 22, 2008)
Experiment Date Start: Jul 24, 2008
Experiment Date End: Jul 26, 2008
Person: Natalia Maltseva
Lab: Argonne National Laboratory
Protocol: standard purification_ANL
Status: success
Description: IMAC-I, SEC::TEV cleavage::concentration and buffer exchange (Amicon 3k or 10k)
Affinity Tag Status: cleavage unsuccessful
SeMet Status: native
Covalent Modification
Final Buffer: 20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT
Final Concentration (mg/mL): 152
Final Volume (μL): 550
Shipped To Lab Id: ANL
Shipped Date: Jul 26, 2008
Inserted: Jul 29, 2011

Related Crystallization Drops

Experiment Date	Person	Lab	Protocol	Status	Method	Screen	Screen Component	Plate Type
Aug 04, 2008	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	ANL-2	44	CrystalQuick standard profile, LBR round bottom plate
Aug 04, 2008	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	30	CrystalQuick standard profile, LBR round bottom plate
Aug 04, 2008	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	24	CrystalQuick standard profile, LBR round bottom plate
Aug 04, 2008	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	32	CrystalQuick standard profile, LBR round bottom plate
Aug 04, 2008	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	13	CrystalQuick standard profile, LBR round bottom plate
Aug 04, 2008	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	5	CrystalQuick standard profile, LBR round bottom plate
Aug 04, 2008	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	72	CrystalQuick standard profile, LBR round bottom plate
Aug 04, 2008	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	69	CrystalQuick standard profile, LBR round bottom plate
Aug 04, 2008	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	ANL-1	76	CrystalQuick standard profile, LBR round bottom plate
Aug 04, 2008	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	ANL-2	72	CrystalQuick standard profile, LBR round bottom plate
Aug 04, 2008	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	ANL-2	22	CrystalQuick standard profile, LBR round bottom plate
Aug 04, 2008	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Wizard Full (I & II)	23	CrystalQuick standard profile, LBR round bottom plate
Aug 04, 2008	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	ANL-2	48	CrystalQuick standard profile, LBR round bottom plate
Aug 04, 2008	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	ANL-2	49	CrystalQuick standard profile, LBR round bottom plate
Aug 04, 2008	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	ANL-2	81	CrystalQuick standard profile, LBR round bottom plate
Aug 04, 2008	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	ANL-2	82	CrystalQuick standard profile, LBR round bottom plate