Purification

Target

IDP00523  

Purification ID

A3521  

Expression

M9 minimal (Jun 08, 2010)  

Experiment Date Start

Jun 16, 2010  

Experiment Date End

Jun 18, 2010  

Person

Min Zhou  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

cleaved  

SeMet Status

native  

Covalent Modification

 

Final Buffer

50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  

Final Concentration (mg/mL)

25  

Final Volume (μL)

1200  

Shipped To Lab Id

ANL  

Shipped Date

Jun 18, 2010  

Inserted

Aug 03, 2011