Center for Structural Genomics of Infectious Diseases

Purification

Target: IDP01750
Purification ID: A5859
Expression: LB (Oct 11, 2010)
Experiment Date Start: Jul 14, 2010
Experiment Date End: Jul 16, 2010
Person: Min Zhou
Lab: Argonne National Laboratory
Protocol: standard purification_ANL
Status: success
Description: IMAC-I, SEC, IMAC-II::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)
Affinity Tag Status: cleaved
SeMet Status: native
Covalent Modification
Final Buffer: 50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole
Final Concentration (mg/mL): 57
Final Volume (μL): 1050
Shipped To Lab Id: ANL
Shipped Date: Jul 16, 2010
Inserted: Aug 03, 2011

Experiment Date	Person	Lab	Protocol	Status	Method	Screen	Screen Component	Plate Type
Jul 21, 2010	Min Zhou	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	94	CrystalQuick standard profile, LBR round bottom plate
Jul 21, 2010	Min Zhou	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	43	CrystalQuick standard profile, LBR round bottom plate
Jul 21, 2010	Min Zhou	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	43	CrystalQuick standard profile, LBR round bottom plate
Jul 21, 2010	Min Zhou	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	72	CrystalQuick standard profile, LBR round bottom plate