Purification

Target

IDP01750  

Purification ID

A4403  

Expression

M9 minimal + semet (Sep 02, 2008)  

Experiment Date Start

Jul 14, 2010  

Experiment Date End

Jul 16, 2010  

Person

Min Zhou  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, SEC, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

cleaved  

SeMet Status

semet  

Covalent Modification

 

Final Buffer

50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  

Final Concentration (mg/mL)

57  

Final Volume (μL)

1050  

Shipped To Lab Id

ANL  

Shipped Date

Jul 16, 2010  

Inserted

Aug 03, 2011