Center for Structural Genomics of Infectious Diseases

Purification

Target: IDP04633
Purification ID: A8640
Expression: M9 minimal (Jan 10, 2012)
Experiment Date Start: Feb 29, 2012
Experiment Date End: Mar 02, 2012
Person: Natalia Maltseva
Lab: Argonne National Laboratory
Protocol: standard purification_ANL
Status: success
Description: IMAC-I, IMAC-II::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)
Affinity Tag Status: cleaved
SeMet Status: native
Covalent Modification
Final Buffer: 20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT
Final Concentration (mg/mL): 26
Final Volume (μL): 2200
Shipped To Lab Id: ANL
Shipped Date: Mar 04, 2012
Inserted: Mar 07, 2012

Related Crystallization Drops

Experiment Date	Person	Lab	Protocol	Status	Method	Screen	Screen Component	Plate Type
Aug 17, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	47	CrystalQuick standard profile, LBR round bottom plate
Mar 02, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	88	CrystalQuick standard profile, LBR round bottom plate
Mar 02, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	94	CrystalQuick standard profile, LBR round bottom plate
Mar 02, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	88	CrystalQuick standard profile, LBR round bottom plate
Mar 02, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	21	CrystalQuick standard profile, LBR round bottom plate
Mar 02, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	42	CrystalQuick standard profile, LBR round bottom plate
Mar 02, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	14	CrystalQuick standard profile, LBR round bottom plate
Mar 02, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	92	CrystalQuick standard profile, LBR round bottom plate
Mar 02, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	18	CrystalQuick standard profile, LBR round bottom plate
Mar 02, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	22	CrystalQuick standard profile, LBR round bottom plate
Mar 02, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	14	CrystalQuick standard profile, LBR round bottom plate
Aug 17, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	14	CrystalQuick standard profile, LBR round bottom plate
Aug 17, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	44	CrystalQuick standard profile, LBR round bottom plate