Center for Structural Genomics of Infectious Diseases

Purification

Target: IDP04364
Purification ID: A8691
Expression: M9 minimal (Sep 14, 2011)
Experiment Date Start: Dec 08, 2011
Experiment Date End: Dec 09, 2011
Person: Natalia Maltseva
Lab: Argonne National Laboratory
Protocol: standard purification_ANL
Status: success
Description: IMAC-I, SEC::SEC peak 1::concentration and buffer exchange (Amicon 3k or 10k)
Affinity Tag Status: uncleaved
SeMet Status: native
Covalent Modification
Final Buffer: 20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT
Final Concentration (mg/mL): 53
Final Volume (μL): 1200
Shipped To Lab Id: ANL
Shipped Date: Dec 10, 2011
Inserted: Mar 09, 2012

Experiment Date	Person	Lab	Protocol	Status	Method	Screen	Screen Component	Plate Type
Feb 28, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Pi-PEG Screen	26	CrystalQuick standard profile, LBR round bottom plate
Feb 28, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Pi-PEG Screen	1	CrystalQuick standard profile, LBR round bottom plate
Feb 28, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Pi-PEG Screen	80	CrystalQuick standard profile, LBR round bottom plate
Feb 28, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Pi-PEG Screen	2	CrystalQuick standard profile, LBR round bottom plate
Feb 28, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Pi-PEG Screen	1	CrystalQuick standard profile, LBR round bottom plate
Feb 28, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Pi-PEG Screen	86	CrystalQuick standard profile, LBR round bottom plate
Feb 28, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Pi-PEG Screen	2	CrystalQuick standard profile, LBR round bottom plate
Feb 28, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Pi-PEG Screen	1	CrystalQuick standard profile, LBR round bottom plate